The Tate Collection's notes include this: "...This portrait shows Elizabeth Roydon at the age of forty and was painted in 1563, as the Latin inscription ‘AETATIS XL, | M.D.LXIII,’ indicates. Elizabeth, who is dressed entirely in black, had recently been widowed, for her second husband, Cuthbert Vaughan, had been killed in that year protecting the French port of Le Havre against French Catholic forces. In the following year she was to marry Sir Thomas Golding of Belchamp St Paul, Essex. The heraldic arms in the top left-hand corner are those of her own family, the Roydons of Kent. They were added some years after the picture was painted, presumably by one of her descendants, to emphasise her status as an heiress in her own right.
To the left of these arms can be seen the conjoined monogram ‘HE’, which is thought to refer to the Antwerp-trained Jan Eeuwowts, known in Britain as ‘Hans Eworth.’ Eworth worked on every scale, from large full-length to minute portrait miniature. Elizabeth Roydon’s portrait is comparatively small in size. It is in extremely good condition for its age and, using very fine brushstrokes, is carried out in a technique similar to that of a miniaturist. The translucency of the paint in the flesh areas means that the freely drawn underdrawing is now visible.
Elizabeth was the daughter of Thomas Roydon (c.1520–c.1571) of Roydon Hall, East Peckham in Kent. She first married, before 1540, William Twysden of Chelmingham in Kent and their eldest son Roger eventually became her principal heir. Elizabeth died on 19 August 1595. Her will shows her to have been a woman of strong religious convictions and an affectionate mother and grandmother who had already given away much plate and jewelry during her lifetime..." Her brief article in thepeerage is here.
From the Google Art Project via liveinternet.ru/users/loreleya-62/post344151039/ and, ultimately, from the Tate Collection. Cracks in the face region were fixed with Photoshop and shadows were filled in.